Hygromycin phosphotransferase gene
WebHPT, the hygromycin phosphotransferase gene. (B) Target sites for eight sgRNAs targeting HD1. The gene structure (upper) and partial abbreviate sequence (lower) of HD1 were shown. The homeodomain and sgRNAs were represented as a black box and arrows, respectively. PAM sites (5′-NGG-3′) were indicated red at the end of each spacer sequence. WebAbstract. Embryogenic maize ( Zea mays L.) callus cultures were transformed by microprojectile bombardment with a chimeric hygromycin phosphotransferase (HPT) …
Hygromycin phosphotransferase gene
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Web21 jan. 2024 · We used embryogenic calli as explant and an empty gRNA-containing CRISPR/Cas9 vector with hptII (hygromycin phosphotransferase) gene for the … WebWe also show that a chimaeric gene consisting of the nopaline synthase (nos) gene regulatory elements and the E. coli derived hygromycin phosphotransferase (hpt) …
Web3 jan. 2024 · To create the ku70 gene deletion construct, 1.9 kb upstream and downstream of the ku70 gene and 0.55 kb of the Y. lipolytica tef1 promoter and the bacterial hygromycin phosphotransferase gene (hph) were amplified by PCR with oligo pairs (Additional file 3, Oligos) 1313/1314 (ku70 upstream), 1315/1321 (hph), 1322/1318 … Web5 mrt. 2024 · This gene cluster also contained a putative transcription factor gene (g432) and a self-resistance gene (g431). Hygromycin B may be used for activation of …
Web潮黴素B(Hygromycin B) 是一種由吸水鏈黴菌（Streptomyces hygroscopicus）產生的抗生素，是氨基糖苷類抗生素之一，為微黃褐色粉末。 潮黴素B可通過抑制 細菌 、 真菌 和一些高等 真核生物 細胞 內的 蛋白質生物合成 來殺死這些生物。 WebSimilarly, expression of the hygromycin B phosphotransferase gene from the transfected vector [e.g., pGL4.14(luc2/Hygro) Vector (Cat.# E6691)] will confer resistance to the drug hygromycin B. An alternative strategy is to use a vector carrying an essential gene that is defective in a given cell line.
WebThe aminoglycoside phosphotransferases achieve inactivation of their antibiotic substrates by phosphorylation. Only phosphorylates hygromycin and closely related …
WebRecord ID: JN113286.1 Description: Solanum tuberosum transgenic clone RH149N09 hygromycin phosphotransferase gene, complete cds. Length: 9769 bp Found in databases: ntemblreltgn 28 amplicons View all records targeted by these methods View these methods in JRC GMO-Matrix long tall sally tomateWebphosphotransferase coding region) gene and a nos 3′ terminator from plasmid p24 was isolated, gel purified and ligated on the 3′-end at the Not I site of pBACKAN with T4 DNA ligase (US long tall sally shoes ukWebSolanum tuberosum transgenic clone RH149N09 hygromycin phosphotransferase gene, complete cds. Taxon: Solanum tuberosum: Original size: 9769 bp: View other 27 matches for this record. Method: QT-ELE-00-004: Go to GMOMETHODS record: View in JRC GMO-Matrix; Description: long tall sally shoes for women size 13WebThe hygromycin phosphotransferase gene (hpt) from E. coli under the control of the SV40 early promoter was used as a dominant selectable marker for transformation … long tall sally the predatorHygromycin is active against both prokaryotic and eukaryotic cells. It acts by inhibiting polypeptide synthesis. It stabilizes the tRNA-ribosomal acceptor site, thereby inhibiting translation. Meer weergeven Hygromycin B is an antibiotic produced by the bacterium Streptomyces hygroscopicus. It is an aminoglycoside that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. Meer weergeven Hygromycin B was originally developed in the 1950s for use with animals and is still added into swine and chicken feed as an anthelmintic or anti-worming agent (product name: … Meer weergeven In the laboratory it is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene. The resistance gene is a kinase that inactivates hygromycin B through phosphorylation. Since the … Meer weergeven long tall sally the beatles lyricsWeb3 mei 2024 · Occurrence of Natural Bacillus thuringiensis Contaminants and Residues of Bacillus thuringiensis-Based Insecticides on Fresh Fruits and Vegetables. PubMed Central. Frederiksen, Kristine; Rosenquist, Hanne; Jørgensen, Kirsten; Wilcks, Andrea. 2006-01-01. A total of 128 Bacillus cereus-like strains isolated from fresh fruits and vegetables for … long tall sally tomatoWebA gene has been identified that confers resistance in E. coli against Hygromycin B. The resistance gene codes for kinase (hygromycin B phosphotransferase) that inactivates … long tall sally track order