Bl539a biosharp
WebJul 30, 2024 · For immunohistochemical studies, tissue samples were immediately fixed in 4% paraformaldehyde (BL539A; Biosharp, Hefei, China) for 24 h. After fixation, the tissues were dehydrated through a … WebDec 3, 2024 · PBS and 4% paraformaldehyde (PFA) (#BL539A, biosharp, China). 2.2 Equipment. Injection syringe: 10 μL microinjection syringes (0.7 mm tip diameter) were …
Bl539a biosharp
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WebDec 12, 2024 · After 72 h of in vitro incubation, the cultures were fixed in paraformaldehyde solution (4% PFA, biosharp, BL539A) for 15 min, washed with PBS, permeabilized with PBS containing 0.3% Triton X-100 for 20 min at room temperature, closed with goat serum (GIBCO, 16210064) for 30 min at room temperature, and stained with Anti-beta III … WebMar 20, 2024 · Abstract Here, we present a protocol for the detection of the two STING isoforms (erSTING and pmSTING) in human peripheral blood mononuclear cells or mouse splenocytes using Western blot and PCR. We detail steps to construct plasmids encoding each isoform and transfer them into mouse and human cell lines.
http://www.biosharp.cn/index/product/details/language/en/product_id/1862.html WebMar 3, 2024 · After drug treatment, cells were gently washed by PBS for three times and fixed with 4% fresh paraformaldehyde-phosphate (BL539A, Biosharp) for 15 min, then permeabilized with 0.5% Triton X-100 in PBS for 10 min at room temperature.
WebTwo proteins, GJA1 and SLC39A14, exist in female blood exosomes and are highly expressed in HMSMCs exosomes, are also upregulated in the laboring myometrium, which verified increased in laboring blood samples, might be novel potential biomarkers for myometrial activation. WebBL539A 4%多聚甲醛/通用型组织固定液 500ml 产品简介： 组织细胞中的大多数肽类激素、蛋白物质为水溶性,在进行病理研究之前必需固定。 多聚 …
WebSep 6, 2024 · Paraffin-embedded sections of 4T1 tumor tissues were fixed with 4% paraformaldehyde (BL539A, Biosharp) for 24 h at a constant temperature. The tumor tissues were then embedded in paraffin and cut into 4-µ m-thick slices and then transferred to glass slides. After dewaxing and hydrating, the slides were placed in citrate buffer (pH …
WebJun 14, 2024 · For histological assessment, the isolated cardia of the gastric tissue was fixed in 4% formaldehyde (BL539A, Biosharp, Anhui, China) (Lin et al. 2024). Then, tissue samples were embedded in paraffin. Paraffin sections were subsequently sliced into thin slices with a thickness of 4 μm. The tissues were dewaxed and subsequently stained with ... burn feet treatmentWebExercise improved learning and memory Nine-week old male mice with or without exercise for 4 weeks were subjected to left carotid artery exposure (surgery) under isoflurane anesthesia (panels A–C). burn feet careWebImmunohistochemical staining Tissues were fixed with 4% paraformaldehyde (BL539A, Biosharp, Shanghai, China) and embedded in paraffin. Next, each section (thickness, 5 … burn fellowshipWebApr 4, 2024 · The results showed that volume and weight of tumors was significantly inhibited by LBH589 treatment (Figure 7A-C). IHC demonstrated that LBH589 inhibited … ham bone vegetable soup slow cookerWeb4%多聚甲醛 通用型组织固定液500ml即用型试剂 BL539A [Biosharp] 钼酸钠 钼酸二钠 分析纯AR500g 分析试剂 银碘含量≥99% 正品包邮 氯化钠 分析纯AR500g 含量≥99.5% 天津光复 高纯分析试剂 正品 burn fencing balneWebJan 15, 2024 · Visual presentation of the LMMP separation process. (A) After cutting the colon into four pieces (3–4 cm in length per segment), a syringe with the needle removed … burn fenceWebAfter culturing for 1 hour, unadhered cells were removed, while the rest cells were fixed with 4% paraformaldehyde (#BL539A, Biosharp, China) and stained with crystal violet (#C0121, Beyotime, China). Photos were taken for statistic al analysis . MKN-45 cell processing was conducted the same as MGC-803 cells. Apoptosis assays ham bone white bean soup